Background and Purpose: Hypoxic-ischemic brain injury, due to reduced supply of oxygen to brain, is a major cause of death and disability. There is no exclusive treatment available so far. Glycine-2-Methylproline-Glutamate (G-2MePE, NNZ 2566), an analogue of Glycine-Proline-Glutamate reduces neuronal injury after focal ischemia in adult rats. The current study investigated into the neuroprotective effects of G-2MePE after global hypoxic-ischemic brain injury in adult rats.Methods: Adult male rats received a single sub cutaneous injection of G-2MePE (1.2mg/kg) 3h post hypoxic-ischemic brain injury or the same volume of normal saline. Brains were extracted 5 days after the treatment. Tissue damage in the cortex, hippocampus and striatum was assessed. Neuronal survival, glial reactions, caspase-3 activity and TNF-α cytokine activity were also assessed.Results: The treatment with G-2MePE was associated with a significant reduction of tissue damage, improvement in neuronal survival, reduction in reactive microglia, TNF-α positive cells and caspase-3 positive cells in hippocampus and cortex but an elevation of astrocytosis.Conclusions: Neuroprotection with G-2MePE after hypoxic-ischemic brain injury in adult rats is associated with reduced neuronal necrosis, apoptosis, modulated inflammatory responses and augmented astrocytosis.
- Build a closely connected, mutually beneficial network of active scientists who are actively doing experimental and translational stroke research;
- Foster exchange of information on stroke models, experimental and translational stroke research among peers;
- Coordinate hands-on training and promote Standard Operational Procedures (SOP) for stroke models;
- Culture an environment that favors identifying and solving problems;
- Improve the quality of experimental stroke research and facilitate a successful translation for ischemic stroke treatment.
The Journal's focus and scopeJESTM provides high quality tailored contents to stroke investigators. The scope of the journal covers original studies and reviews in experimental stroke and translational medical research. Special emphases are placed on new concepts and innovative research ideas in neuroprotection for acute ischemic stroke, and methodological improvements in stroke studies. JESTM differs from other stroke journals in the following aspects:
- It encourages authors to submit articles with controversial/provocative ideas and negative results;
- A unique article type "Innovative Research Idea" is available for accepting innovative research ideas, which is something similar to the abbreviated scientific part of a research proposal;
- It accepts improved protocols;
- The journal site provides a networking platform for building up professional connections;
- It is both free and open-access.